Figure 4

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Figure 4. Automated analysis and troubleshooting within the HTAPP LC/MS data acquisition module. A) Designation of automated SEQUEST search and database deposition parameters for proteomic samples; B) Post-acquisition data pusher for initiation of autonomous post-acquisition analysis; C) Thresholds set for automated real-time monitoring of selected ion chromatogram peak heights or areas of bovine serum albumin (BSA), alpha casein peptides and user-selected masses in users' proteomic samples that triggers email alerts and/or halts the automated acquisition queue; D) Historical archive of HPLC gradient and pressure profile monitoring for multiple pumps displayed in a web browser; E) A webpage to monitor the live running status of LC/MS; F) Historical archive of three selected BSA peptide ion chromatogram peak areas from automated standard runs.